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• Vol.49 No.11
• CLINICA NEUROL, 49: 910−912, 2009
• Vol.49 No.11 contents

The 50th Annual Meeting of the Japanese Society of Neurology

Genetic approach for understanding of the late-onset mechanisms of polyglutamine disease

Erina Kuranaga¹⁾²⁾, Ayako Tonoki¹⁾²⁾ and Masayuki Miura¹⁾²⁾

¹⁾Department of Genetics, Graduate School of Pharmaceutical Sciences, University of Tokyo
²⁾CREST, JST

The intracellular accumulation of unfolded or misfolded proteins is believed to contribute to aging and age-related neurodegenerative diseases. However, the links between age-dependent proteotoxicity and cellular protein degradation systems remain poorly understood. Here, we show that 26S proteasome activity and abundance attenuate with age, which is associated with the impaired assembly of the 26S proteasome with the 19S regulatory particle (RP) and the 20S proteasome. In a genetic gain-of-function screen using Drosophila, we characterized Rpn11, which encodes a subunit of the 19S RP, as a suppressor of expanded polyglutamine-induced progressive neurodegeneration. Rpn11 overexpression suppressed the age-related reduction of the 26S proteasome activity, resulting in the extension of flies' life spans with suppression of the age-dependent accumulation of ubiquitinated proteins. On the other hand, the loss of function of Rpn11 caused an early onset of reduced 26S proteasome activity and a premature age-dependent accumulation of ubiquitinated proteins. It also caused a shorter life span and an enhanced neurodegenerative phenotype. Our results suggest that maintaining the 26S proteasome with age could extend the life span and suppress the age-related progression of polyglutamine diseases.
Full Text of this Article in Japanese PDF (458K)

(CLINICA NEUROL, 49: 910−912, 2009)
key words: Genetic screening, Ubiquitin-Proteasome system, Drosophila

(Received: 22-May-09)